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Related Concept Videos

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

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Related Experiment Video

Updated: Jul 7, 2026

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
09:03

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Published on: February 7, 2012

Signalling pathways regulating cardiac neural crest migration and differentiation.

Frances High1, Jonathan A Epstein

  • 1Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.

Novartis Foundation Symposium
|February 28, 2008
PubMed
Summary

Advanced mouse genetics reveals new molecular pathways regulating cardiac neural crest development. These findings identify novel genes involved in congenital heart disease, particularly during aortic arch remodeling.

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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

Related Experiment Videos

Last Updated: Jul 7, 2026

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
09:03

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Published on: February 7, 2012

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
12:48

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Published on: June 2, 2012

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
09:33

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

Area of Science:

  • Cardiovascular development
  • Developmental biology
  • Molecular genetics

Background:

  • The neural crest is crucial for cardiovascular system development.
  • Recent advances in mouse genetics have uncovered new molecular pathways governing cardiac neural crest cell (CNCC) migration and differentiation.
  • Understanding these pathways is vital for addressing congenital heart disease.

Purpose of the Study:

  • To elucidate novel molecular mechanisms regulating cardiac neural crest migration and differentiation.
  • To identify candidate genes for congenital heart disease based on neural crest and cardiovascular patterning roles.

Main Methods:

  • Utilized advanced mouse genetic techniques.
  • Investigated molecular pathways involving bone morphogenetic protein (BMP), T-box, myocardin, Gata, and Notch families.
  • Examined the role of axon guidance molecules, including semaphorins, plexins, and neuropilins, in cardiovascular patterning.

Main Results:

  • Identified previously unrecognized molecular pathways regulating CNCC migration and differentiation.
  • Demonstrated the involvement of BMP, T-box, myocardin, Gata, and Notch signaling families.
  • Highlighted the critical roles of semaphorin, plexin, and neuropilin families in aortic arch remodeling.
  • Implicated these molecules as candidate genes contributing to congenital heart disease.

Conclusions:

  • Advanced mouse genetics has unveiled novel molecular regulators of cardiac neural crest development.
  • Semaphorin-plexin-neuropilin signaling is critical for aortic arch remodeling and implicated in congenital heart disease.
  • These findings provide new insights into the genetic basis of congenital heart defects.