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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jul 7, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.

Sandra L Spurgeon1, Robert C Jones, Ramesh Ramakrishnan

  • 1Fluidigm Corporation, South San Francisco, California, USA.

Plos One
|February 28, 2008
PubMed
Summary

A new microfluidic dynamic array system enables 2,304 simultaneous real-time PCR gene expression measurements. This high throughput platform offers improved reproducibility and reduced pipetting for gene expression analysis.

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Last Updated: Jul 7, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Published on: April 14, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Gene expression profiling is crucial for understanding biological processes.
  • High throughput methods are needed to analyze large numbers of genes and samples efficiently.
  • Current platforms can be limited by throughput, reproducibility, or sample volume.

Purpose of the Study:

  • To introduce and validate a novel microfluidic dynamic array platform for gene expression analysis.
  • To demonstrate the system's capacity for high throughput and multiplexed real-time PCR.
  • To compare the performance of the microfluidic system against conventional methods.

Main Methods:

  • Development of a microfluidic dynamic array chip capable of performing 2,304 real-time PCR reactions.
  • Simultaneous measurement of 45 different genes across 18 tissue types with replicates on a single chip.
  • Comparison of data generated by the microfluidic system with conventional real-time PCR and commercial DNA microarrays.

Main Results:

  • The microfluidic dynamic array system successfully performed 2,304 simultaneous gene expression measurements.
  • The platform requires less pipetting compared to traditional 96-well plate setups.
  • Data from the microfluidic system showed excellent concordance with conventional real-time PCR.
  • Microfluidic dynamic arrays demonstrated superior reproducibility compared to commercial DNA microarrays.

Conclusions:

  • Microfluidic dynamic arrays provide a powerful, high throughput platform for gene expression analysis.
  • The system offers enhanced reproducibility and efficiency for large-scale gene expression studies.
  • This technology has the potential to advance research in various fields requiring detailed gene expression profiling.