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Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
Published on: November 25, 2017
Mannose accommodation of Vigna angularis cells on solid agar medium involves its possible conversion to sucrose
1Biology and Environmental Sciences, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, 790-8577, Japan.
Journal of Plant Research
|February 28, 2008
Summary
Azuki bean cells cannot use mannose as a carbon source, inhibiting growth. However, callus cultures adapt to mannose by enhancing phosphomannose isomerase activity for sucrose conversion.
Area of Science:
- Plant Physiology
- Plant Cell Culture
- Biochemistry
Background:
- Mannose is generally not a usable carbon source for many plant species.
- Understanding plant carbon metabolism is crucial for crop improvement and biotechnology.
Purpose of the Study:
- To investigate the effects of mannose versus sucrose on azuki bean (Vigna angularis) cell growth and intracellular sucrose levels.
- To explore the adaptation mechanisms of plant cells to mannose as a carbon source.
Main Methods:
- Comparison of azuki bean suspension cells grown in liquid media with sucrose or mannose.
- Analysis of intracellular sucrose levels and phosphomannose isomerase activity.
- Induction and characterization of mannose-acclimated callus cultures from suspension cells.
Main Results:
- Suspension cells grew in sucrose but not mannose, with significantly reduced intracellular sucrose levels in mannose.
- Callus cultures developed from suspension cells after a lag period on mannose-containing medium.
- Mannose-acclimated callus cells showed enhanced phosphomannose isomerase activity, enabling mannose to sucrose conversion.
Conclusions:
- Limited conversion of mannose to sucrose inhibits growth in azuki bean suspension cells.
- Callus culture facilitates adaptation to mannose utilization through enhanced enzymatic activity.
- A distinct difference in metabolic capabilities exists between suspension and callus cultures regarding mannose metabolism.
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