Japanese encephalitis virus envelope protein mitigates TNF-alpha mRNA expression in RAW264.7 cells

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Tsai-Ching Hsu1, Ji-Qiang Gao, Ko-Hsiu Lu

  • 1Institute of Immunology, Chung Shan Medical University, Taichung, Taiwan, Republic of China.

Inflammation
|February 28, 2008
PubMed

Insights

Japanese encephalitis virus envelope protein (JEVE) may reduce TNF-alpha mRNA expression in monocytes. This effect is linked to increased p53-independent p21(Waf1/Cip1) expression, potentially explaining JEV transmission via monocytes.

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Japanese encephalitis virus (JEV) is a significant mosquito-borne pathogen causing severe illness.
  • Monocytes play a role in JEV transmission, but the impact of the JEV envelope (JEVE) protein on monocytes is poorly understood.

Purpose of the Study:

  • To investigate the effects of the JEVE protein on monocyte function.
  • To elucidate the molecular mechanisms underlying JEVE's influence on cellular responses relevant to JEV infection.

Main Methods:

  • RAW264.7 cells were transfected with plasmids expressing either JEVE or a control (pEGFP).
  • Quantitative analysis of tumour necrosis factor (TNF)-alpha mRNA, p21(Waf1/Cip1), p53, and nuclear factor-kB (NF-kB) expression was performed.

Main Results:

  • Transfection with pEGFP-JEVE significantly decreased TNF-alpha mRNA expression compared to pEGFP controls.
  • p21(Waf1/Cip1) protein increased in both pEGFP and pEGFP-JEVE transfected cells.
  • p53 protein increased only in pEGFP-transfected cells, and NF-kB expression was not elevated in pEGFP-JEVE transfected cells.

Conclusions:

  • JEVE protein alleviates TNF-alpha mRNA expression in monocytes.
  • This effect is associated with increased p53-independent p21(Waf1/Cip1) expression.
  • Findings provide insights into JEV transmission mechanisms involving monocytes.

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