Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Condensins02:15

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coordinated chromosome motion emerges from mechanical coupling mediated by the physical spindle environment.

Molecular biology of the cell·2026
Same author

Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2026
Same author

Three types of actomyosin rings within a common cytoplasm exhibit distinct modes of contractility.

Molecular biology of the cell·2025
Same author

Animal septins contain functional transmembrane domains.

Current biology : CB·2025
Same author

Three types of actomyosin rings within a common cytoplasm exhibit distinct modes of contractility.

bioRxiv : the preprint server for biology·2024
Same author

Animal septins contain functional transmembrane domains.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Jul 7, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Chromosome segregation: centromeres get bent.

Jonas F Dorn1, Paul S Maddox

  • 1Department of Pathology and Cell Biology, Université de Montreal, Montréal, Quebec H3C 3J7, Canada.

Current Biology : CB
|February 28, 2008
PubMed
Summary

Intercentromeric chromatin actively participates in chromosome segregation, challenging the long-held focus on kinetochores. This research highlights DNA

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Kinetochores are traditionally recognized for their active role in chromosome segregation.
  • The involvement of chromatin and DNA in this process has been largely overlooked.

Purpose of the Study:

  • To investigate the role of intercentromeric chromatin in chromosome segregation.
  • To re-evaluate the contribution of DNA and chromatin to accurate cell division.

Main Methods:

  • The study analyzed findings from two independent research groups (Bloom and Khodjakov).
  • The research likely involved advanced microscopy and genetic manipulation techniques (details not provided in abstract).

Main Results:

  • Intercentromeric chromatin demonstrates a significant, active role in chromosome segregation.

More Related Videos

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana
07:18

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana

Published on: October 24, 2025

Related Experiment Videos

Last Updated: Jul 7, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana
07:18

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana

Published on: October 24, 2025

  • This finding contrasts with the historical emphasis on kinetochore function.

    • Conclusions:

    • Chromatin, particularly intercentromeric regions, is a crucial player in chromosome segregation.
    • Future research should consider the dynamic role of DNA and chromatin in maintaining genomic stability.