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Updated: Jul 7, 2026
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
C/EBPalpha S248A mutation reduces granulocytic differentiation in human leukemic K562 cells
Sheo M Singh1, A K Trivedi, Gerhard Behre
1Bone Marrow Transplantation Section, State Center for Cell and Gene Therapy, Department of Internal Medicine IV, Martin-Luther-University, Ernst Grube Str. 40, Halle 06097, Germany.
Abstract:
Phosphorylation of C/EBPalpha can either lead to granulocytic differentiation or a block in granulopoiesis. This dichotomy in effect is dependent on the upstream signaling pathway and the phosphorylation site in C/EBPalpha. Ras signaling induced phosphorylation of S248 residue of C/EBPalpha is known to enhance granulocytic differentiation. In this study, using beta-estradiol inducible stable cell lines we show that the point mutation of phosphorylation site S248 in C/EBP disrupts the CD11b and GCSFr expression and subsequently reduces the differentiation of leukemic K562 cells. Based on our observations in the present study, we conclude that S248A mutation of C/EBPalpha leads to a reduction of granulocytic differentiation markers and a block in differentiation at the morphological level.
