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[Effect of anti-BPDE on expression of HER2/neu gene in the human bronchial epithelial cell line]

Juan Fu1, Yiguo Jiang, Yunfeng Zou

  • 1Department of Occupational and Environmental Health, MOE Key Lab of Environment and Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. fjuangy@126.com

Wei Sheng Yan Jiu = Journal of Hygiene Research
|February 29, 2008
PubMed
Abstract

Insights

Exposure to anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti-BPDE) significantly increases HER2/neu gene expression in human bronchial cells. This overexpression may contribute to malignant transformation.

Area of Science:

  • Environmental Toxicology
  • Molecular Biology
  • Oncology

Context:

  • Benzo(a)pyrene is a common environmental carcinogen.
  • HER2/neu gene amplification is implicated in various cancers.
  • Human bronchial epithelial cells are targets for environmental mutagens.

Purpose:

  • To investigate the impact of anti-BPDE on HER2/neu gene expression.
  • To analyze HER2/neu mRNA and protein levels in response to anti-BPDE.
  • To determine the cellular localization of HER2/neu protein.

Summary:

  • Treatment of human bronchial epithelial cells with anti-BPDE led to a significant increase in HER2/neu mRNA and protein expression.
  • Quantitative RT-PCR, western blot, and immunocytochemistry confirmed HER2/neu overexpression.
  • HER2/neu protein was observed to localize to the cell membrane.

Impact:

  • Results suggest that anti-BPDE can induce HER2/neu overexpression in bronchial cells.
  • HER2/neu oncogene activity may play a role in anti-BPDE-induced malignant transformation.
  • This finding contributes to understanding the molecular mechanisms of chemical carcinogenesis.

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