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Updated: Jul 7, 2026

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
Published on: August 7, 2017
Isolation and potential existence of side population cells in adult human kidney
Toshihiko Inowa1, Keiichi Hishikawa, Takumi Takeuchi
1Department of Urology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
Abstract:
The existence of adult stem-like cells such as side population (SP) cells is reported in various kinds of animal tissues, and we recently reported that mice kidney SP cells differentiate into multilineage. However, there has thus far been no report about human kidney SP cells. In the present study, we examined the existence of SP cells in human kidney tissue by using Hoechst 33342 staining and fluorescence-activated cell sorting analysis. We used porcine kidney tissue to optimize the analysis conditions for human tissue, and found that the SP population in human kidney was 1.3%. The existence of SP cells in human kidney suggests that the cells could be good targets for clinical renal regenerative medicine.
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