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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

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Three PIGGYBACK genes that specifically influence leaf patterning encode ribosomal proteins.

Violaine Pinon¹, J Peter Etchells, Pascale Rossignol

¹Department of Crop Genetics, John Innes Centre, Norwich NR4 7UH, UK.

Development (Cambridge, England)

|February 29, 2008

Summary

This summary is machine-generated.

Three new genes, PIGGYBACK (PGY1-3), impact leaf patterning by interacting with key polarity pathways. These ribosomal proteins suggest translation regulates plant development.

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Area of Science:

Plant Biology
Developmental Biology
Genetics

Background:

Leaves develop polarity through gene interactions specifying adaxial (dorsal) and abaxial (ventral) fates.
ASYMMETRIC LEAVES1 (AS1) is a transcription factor involved in leaf polarity, but other pathways exist, especially in Arabidopsis.

Purpose of the Study:

To identify novel genes involved in leaf patterning and dorsoventral polarity in Arabidopsis.
To understand the genetic interactions regulating leaf development beyond the known AS1 pathway.

Main Methods:

Mutagenesis screens in an as1 mutant background to identify genes affecting leaf patterning.
Genetic analysis, including epistasis tests with known polarity genes like REVOLUTA (REV) and KANADI (KAN).
Identification of the molecular function of the identified genes.

Main Results:

Three new genes, PIGGYBACK1 (PGY1), PGY2, and PGY3, were identified; their mutants exhibit ectopic outgrowths on the adaxial leaf side in an as1 background.
The leaf patterning defects were modulated by mutations in HD-ZIPIII (REV) and KANADI genes, indicating interaction with this pathway.
PGY1, PGY2, and PGY3 encode ribosomal proteins (RPL10a, RPL9, and RPL5), suggesting a role for translation in leaf polarity.

Conclusions:

The PGY genes are crucial regulators of leaf dorsoventral patterning, interacting with the established HD-ZIPIII-KANADI genetic network.
This study reveals a novel role for translation and ribosomal proteins in controlling key plant developmental patterning events.