Effects of lead exposure on sperm concentrations and testes weight in male rats: a meta-regression analysis

The correlation between exposure to lead (PB) and sperm concentrations and testes weight are important in risk assessment of metal toxicity to male reproductive functions. The authors carried out two systematic reviews and meta-analyses of rats. In addition, a meta-regression analysis was taken to explore a dose-response relationship between the mean difference of testes weight (MD(testes)) and available confounding factors. Data were obtained from computerized literature searches of several databases from their inception through December 2005. The reference lists of identified articles were examined further for relevant articles. The authors identified 6 and 12 studies, separated into 16 and 28 subgroups, in the two meta-analyses. The authors conducted random- or fixed-effect models toward the effect size. Heterogeneity between study results was explored through chi-square tests and meta-regression. Results showed that a decrease in sperm concentrations was found as low as mean difference of sperm concentrations (MD(sperm)) = 30.9 and 95%CI = (25.43-36.37) in a fixed-effect model or MD(sperm) = 35.47 and 95%CI = (15.27-55.68) in a random-effect model after Pb exposure. Similarly, a significantly lower testes weight was also evident: MD(testes) = 0.033 and 95%CI = (0.021-0.046) in a fixed-effect model or MD(testes) = 0.047 and 95%CI = (0.0044-0.089) in a random-effect model. In the meta-regression analysis, two confounders, age and body weight, explained part of the observed heterogeneity. The body weight after Pb exposure was inversely associated with MD(testes). These findings support the notion that Pb exposure produced decreased sperm concentrations and testes weight in rats.