Serological studies of Porphyromonas (Bacteroides) gingivalis and correlation with enzyme activity

Porphyromonas gingivalis from the human oral cavity was serologically characterized using absorbed and unabsorbed rabbit antisera. The reference strains were ATCC 33277, W50, W83, 381 and hara 1. The 432 isolates were from periodontal pockets of 63 patients with adult periodontitis. Using sonicated antigens, four serotypes were identified by immunodiffusion tests and immunoelectrophoresis. Each patient harbored only one serotype of P. gingivalis, and serotypes I and IV predominated. The incidence of serotype I was four times higher than that of serotype II, and approximately seven times higher than that of serotype III. The collagenolytic and some proteolytic enzymes of representatives of each serotype were assessed. Although all strains produced these enzymes to some degree, some differences in their levels were observed. Serotype II strains were more collagenolytic than serotypes I or III, and serotype III exhibited lower activities of N-CBz-glycyl-glycyl-arginyl peptidase than other serotypes. Antibiotic sensitivity was also compared with antimicrobial disks, and serotype IV strains exhibited high sensitivity to the four antibiotics used.