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Updated: Jul 7, 2026

Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
Published on: May 15, 2021
Hydration of microcrystalline cellulose and milled cellulose studied by sorption calorimetry
Vitaly Kocherbitov1, Stefan Ulvenlund, Maria Kober
1Biomedical Laboratory Science and Technology, Faculty of Health and Society, Malmö University, SE-205 06 Malmö, Sweden. Vitaly.Kocherbitov@hs.mah.se
Abstract:
The hydration of two different polymorphs of microcrystalline cellulose (cellulose I and II), as well as the hydration of amorphous cellulose was studied using water sorption calorimetry, gravimetric water vapor sorption, nitrogen sorption, and X-ray powder diffraction. Amorphous cellulose was prepared by means of ball-milling of microcrystalline cellulose (MCC). Whereas X-ray data showed the untreated MCC to consist of cellulose I, the amorphous cellulose was found to recrystallize into cellulose II after contact with water or water vapor at relative humidities (RHs) above 90%. Sorption isotherms show an increase of water sorption in the sequence cellulose I
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