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Updated: Jul 7, 2026

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Published on: July 8, 2021
[Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1]
Li-An Wu1, Ling-Ying Wen, Fu-Sheng Yang
1Department of Pediatric Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Objective:
To investigate the regulation effects of upstream stimulatory factor 1 (USF1) on osteopontin expression in odontoblasts.
Methods:
Odontoblast MDPC-23 was cultured and stably transfected with PCMV-USF1 or A-USF plasmids. Total RNA was extracted and osteopontin expression examined by semi-quantitative RT-PCR. Gray value of osteopontin was measured and statistic analysis performed.
Results:
Clones of stable PCMV-USF1 and A-USF plasmids transfection were obtained. Compared with the control, osteopontin was upregulated in PCMV-USF1 transfection group, and downregulated in A-USF transfection group.
Conclusions:
Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts, which could be blocked partly by A-USF.
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