Related Experiment Videos
Link between low-dose environmentally relevant cadmium exposures and asthenozoospermia in a rat model
Susan Benoff1, Karen Auborn, Joel L Marmar
1Fertility Research Laboratories, The Feinstein Institute for Medical Research, Manhasset, New York 11030, USA. Sbenoff@nshs.edu
Objective:
To define the mechanism(s) underlying an association between asthenozoospermia and elevated blood, seminal plasma, and testicular cadmium levels in infertile human males using a rat model of environmentally relevant cadmium exposures.
Setting:
University medical center andrology research laboratory.
Animal(S):
Male Wistar rats (n = 60), documented to be sensitive to the testicular effects of cadmium.
Intervention(S):
Rats were given ad libitum access to water supplemented with 14% sucrose and 0 mg/L, 5 mg/L, 50 mg/L, or 100 mg/L cadmium for 1, 4, or 8 weeks beginning at puberty.
Main Outcome Measure(S):
Testicular cadmium levels were determined by atomic absorption, cauda epididymal sperm motility by visual inspection, and testicular gene expression by DNA microarray hybridization.
Result(S):
Chronic, low-dose cadmium exposures produced a time- and dose-dependent reduction in sperm motility. Transcription of genes regulated by calcium and expression of L-type voltage-dependent calcium channel mRNA splicing variants were altered by cadmium exposure. Expression of calcium binding proteins involved in modulation of sperm motility was unaffected.
Conclusion(S):
A causal relationship between elevated testicular cadmium and asthenozoospermia was identified. Aberrrant sperm motility was correlated with altered expression of L-type voltage-dependent calcium channel isoforms found on the sperm tail, which regulate calcium and cadmium influx.
