[Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua]
Jian-Qiang Kong1, Wei Wang, Xiao-Dong Zheng
1Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Objective:
Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua.
Method:
A P450 cDNA gene was cloned from A. annua by RT-PCR. The bioinformatics analysis of the P450 gene was performed.
Result:
The complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa. The sequence was reported to GenBank and coded as DQ667171. Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54. 992 kDa, it's isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2.
Conclusion:
The comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.
