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The pyruvate kinase-coupled assay for ATPases: a critical analysis
1Department of Chemistry, Indiana University, Bloomington 47405.
Analytical Biochemistry
|April 1, 1991
Abstract:
The pyruvate kinase assay for ATPases suffers from major systematic errors due to the variable amounts of the inhibitory product, ADP, present as the rates change. A simple method is presented to estimate the true uninhibited rates and the ADP inhibition constant for the ATPase.