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Related Experiment Videos

Oligonucleotide fingerprinting using simple repeat motifs: a convenient, ubiquitously applicable method to detect

J T Epplen1, H Ammer, C Epplen

  • 1Max-Planck-Institute for Psychiatry, Martinsried, Germany.

EXS
|January 1, 1991
PubMed
Summary

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Simple repetitive oligonucleotide probes enable multilocus DNA fingerprinting across diverse species, including humans for individual identification and paternity testing. These probes are valuable in forensic science and tumor analysis, offering advantages over PCR.

Area of Science:

  • Molecular Biology
  • Genetics
  • Forensic Science

Background:

  • Multilocus DNA fingerprinting utilizes simple repetitive oligonucleotide probes.
  • These probes have been tested across fungal, plant, animal, and human species.
  • The human genome has been a primary focus for DNA fingerprinting applications.

Purpose of the Study:

  • To design and test simple repetitive oligonucleotide probes for multilocus DNA fingerprinting.
  • To assess the informativeness of these probes across a wide range of species.
  • To explore applications in human individualization, paternity analysis, forensic science, and tumor profiling.

Main Methods:

  • Design and application of a panel of simple repetitive oligonucleotide probes.
  • Testing probe informativeness in approximately 200 species.

Related Experiment Videos

  • Isolation of locus-specific probes from human fingerprints, including hypervariable markers.
  • Scrutiny of a mixed simple repeat locus within the HLA-DRB genes.

    • Main Results:

    • At least one probe was informative in every species tested.
    • Probes (CAC)5 or (GTG)5 allow individualization of humans, except monozygotic twins.
    • Multilocus fingerprints are routinely used for paternity analyses and forensic stain analysis.
    • Tumor fingerprint patterns can reveal chromosomal changes.
    • Hypervariable mono-locus probes were successfully isolated.
    • Significant allelic variation was found in the HLA-DRB gene intron.

    Conclusions:

    • Simple repetitive oligonucleotide probes are versatile tools for DNA fingerprinting in diverse organisms.
    • These probes have practical applications in forensics, paternity testing, and cancer research.
    • They offer advantages over existing methods like PCR and are useful for genomic library ordering.