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Related Concept Videos

Biofilms01:29

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

Correlation between Enterococcus faecalis biofilms development stage and quantitative surface roughness using atomic

Ricardo P Santos1, Theodora T P Arruda, Cibele B M Carvalho

  • 1Laboratório de Ciência e Tecnologia de Materiais, Departamento de Física, Universidade Federal do Ceará, Caixa Postal 6030, Campus do Pici, 60455-900 Fortaleza, Ceará, Brazil. rpsantos2000@yahoo.com

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|March 4, 2008
PubMed
Summary

This study used atomic force microscopy (AFM) to observe topographical changes in Enterococcus faecalis biofilms over time. Increased surface roughness correlated with biofilm development stages, revealing insights into bacterial surface characteristics.

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Quantifying Elastic Properties of Environmental Biofilms using Optical Coherence Elastography
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Quantifying Elastic Properties of Environmental Biofilms using Optical Coherence Elastography

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Quantifying Elastic Properties of Environmental Biofilms using Optical Coherence Elastography
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Quantifying Elastic Properties of Environmental Biofilms using Optical Coherence Elastography

Published on: March 1, 2024

Area of Science:

  • Microbiology
  • Surface Science
  • Biophysics

Background:

  • Biofilms are microbial communities crucial in various processes like biofouling, corrosion, and drug resistance.
  • Understanding biofilm morphology is vital for addressing these implications.
  • Atomic force microscopy (AFM) is a powerful tool for examining biofilm development on surfaces.

Purpose of the Study:

  • To investigate topographical changes during Enterococcus faecalis biofilm development.
  • To characterize bacterial surface aspects and biofilm stages using AFM.
  • To correlate surface roughness with biofilm development.

Main Methods:

  • Enterococcus faecalis biofilms were grown on cellulose nitrate membrane filters in BHI broth.
  • Biofilms were analyzed using AFM at various time points (24, 36, 72, 192, and 360 hours).
  • AFM height images were used to assess topographical changes and surface roughness (mean roughness).

Main Results:

  • AFM revealed topographical changes consistent with biofilm development.
  • Extracellular polymeric substances were identified on the bacterial surface.
  • A correlation was observed between biofilm development stages and increased surface roughness.

Conclusions:

  • AFM effectively visualizes topographical changes during biofilm formation.
  • Surface roughness, quantified by mean roughness, serves as an indicator of biofilm development stage.
  • This study provides insights into the structural evolution of Enterococcus faecalis biofilms.