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Updated: Jul 7, 2026
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
Published on: January 16, 2019
A dual-purpose synthetic colloidal platform for protease mapping: substrate profiling for Dengue and West Nile virus
Lionel Marcon1, Darby Kozak, Bronwyn J Battersby
1Nanotechnology and Biomaterials Centre, Level 5 East, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Saint Lucia, QLD 4072, Australia. l.marcon@uq.edu.au
Abstract:
In a proof of concept study, we created a small focused fluorescent hexapeptide library onto 14 multiplexed barcoded sets of silica particles to probe the substrate recognition specificity of West Nile and Dengue virus proteases. A flow cytometric analysis demonstrated that the optical signature of each bead population remained distinguishable throughout the solid-phase peptide synthesis and proteolytic assay. As expected, both proteases displayed a narrow specificity for lysine and arginine residues in the P(1) and P(2) substrate positions. This open-ended platform enables the fast and simultaneous identification of peptide substrates and is applicable to other proteases.
