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Related Experiment Video

Updated: Jul 7, 2026

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

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Published on: September 11, 2015

Single-molecule diffusion study of activated EGFR implicates its endocytic pathway.

Zeyu Xiao¹, Wei Zhang, Yong Yang

¹Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.

Biochemical and Biophysical Research Communications

|March 4, 2008

Summary

Epidermal growth factor receptor (EGFR) lateral diffusion reveals its internalization pathways. Activated EGFR associates with clathrin-coated pits and caveolae depending on epidermal growth factor (EGF) dose, indicating dual endocytosis mechanisms.

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Area of Science:

Cell biology
Molecular biology
Biophysics

Background:

Epidermal growth factor receptor (EGFR) signaling is crucial for cell growth and is implicated in cancer.
EGFR internalization is a key regulatory step, but its precise pathway remains under investigation.
Clathrin-coated pits and caveolae are known endocytic microdomains involved in receptor internalization.

Purpose of the Study:

To investigate the lateral diffusion of activated EGFR on the cell membrane.
To determine the role of clathrin-coated pits and caveolae in EGFR internalization pathways.
To elucidate how EGF concentration affects EGFR localization within endocytic microdomains.

Main Methods:

Live-cell imaging of individual EGFR molecules using single-particle tracking.
Comparison of EGFR diffusion in intact cells versus cells with disrupted clathrin-coated pits or caveolae.
Manipulation of epidermal growth factor (EGF) concentrations to study dose-dependent effects.

Main Results:

Activated EGFR exhibits distinct lateral diffusion patterns on the cell membrane.
At low EGF doses, activated EGFR preferentially associates with clathrin-coated pits.
At high EGF doses, activated EGFR localizes to both clathrin-coated pits and caveolae.

Conclusions:

EGFR internalization occurs through both clathrin-dependent and caveolae-dependent pathways.
The specific endocytosis pathway utilized by EGFR is dependent on the concentration of its ligand, EGF.
These findings provide critical insights into the regulation of EGFR signaling and potential therapeutic targets.