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Diagnostic value of the total antioxidant capacity (TAC) in human seminal plasma
Reda Mahfouz1, Rakesh Sharma, Dipika Sharma
1Reproductive Research Center, Glickman Urological & Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
Objective:
To establish cutoff value, sensitivity, specificity and intra- and interobserver variability of total antioxidant capacity (TAC) in seminal plasma from healthy donors (controls) and infertile patients.
Design:
Seminal plasma from proven fertile donors (n = 55), nonproven fertile donors (n = 45), and infertile patients (n = 42) were examined for TAC level.
Setting:
Reproductive research center in a tertiary care hospital.
Patient(S):
Infertile patients from male infertility clinic of various diagnoses.
Intervention(S):
Seminal plasma TAC measurement by a colorimetric assay using the TAC assay kit, receiver operating characteristics curve.
Main Outcome Measure(S):
Seminal plasma TAC levels, cutoff value, sensitivity, and specificity.
Result(S):
Proven fertile donors showed higher TAC values (median and range): 1700 (1440-2290 microM); compared with the infertile patients: 1310 (1040-1600 microM). The best cutoff to distinguish between fertile controls and infertile men was 1420 microM. At this threshold, specificity was 64% and sensitivity 76%.
Conclusion(S):
Total antioxidant capacity of the seminal plasma as measured by the colorimetric assay is a reliable and simple test for the diagnosis and management of male infertility.
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