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Updated: Jul 7, 2026

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
Published on: April 11, 2021
Analysis of mitochondrial protein synthesis in yeast
Soledad Funes1, Johannes M Herrmann
1Institut für Physiologische Chemie, Ludwig-Maximilians-Universität München, Germany.
Abstract:
Mitochondrial biogenesis is an intricate process that requires the coordinated function of two separate genetic systems: one in the organelle and one in the nucleus. The study of mitochondria requires the analysis of both genetic systems and their protein products. We describe the general procedures used to label mitochondrially encoded proteins in the baker's yeast Saccharomyces cerevisiae, a starting point for the investigation of various aspects of organelle biogenesis, such as folding and assembly, sorting, and degradation of proteins.
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