Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MitoCatch directs mitochondria delivery and prevents cell degeneration.

Cell research·2026
Same author

Fibroblastic aspartoacylase suppresses TGFβ-mediated responses and cancer progression.

Nature communications·2026
Same author

Mitochondrial calcium uptake drives organelle remodeling to promote inflammasome-dependent cytokine release.

Cell death and differentiation·2026
Same author

Branching out takes anti-tumor immunity down a NOTCH.

Nature immunology·2026
Same author

Cardiolipin preserves T<sub>reg</sub> metabolic fitness and immune homeostasis in the gut.

Nature metabolism·2026
Same author

FAS-controlled T cells drive lymphoproliferation through glycolysis without effector differentiation.

Journal of human immunity·2026

Related Experiment Video

Updated: Jul 7, 2026

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs
08:15

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Measuring mitochondrial shape changes and their consequences on mitochondrial involvement during apoptosis.

Christian Frezza1, Sara Cipolat, Luca Scorrano

  • 1Dulbecco-Telethon Institute, Venetian Institute of Molecular Medicine, Padova, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2008
PubMed
Summary

Mitochondria

More Related Videos

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
06:26

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Published on: April 1, 2011

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

Related Experiment Videos

Last Updated: Jul 7, 2026

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs
08:15

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
06:26

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Published on: April 1, 2011

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

Area of Science:

  • Cell Biology
  • Biochemistry

Background:

  • Mitochondria are central to intrinsic and extrinsic cell death pathways.
  • Mitochondrial dysfunction leads to the release of cytochrome-c, activating caspases.
  • The role of mitochondria-shaping proteins in apoptosis is a key research area.

Purpose of the Study:

  • To investigate how mitochondrial reticulum morphology influences cytochrome-c release.
  • To quantify cytochrome-c release using an in situ approach.
  • To measure real-time changes in mitochondrial membrane potential.

Main Methods:

  • Quantitative in situ analysis of cytochrome-c release.
  • Real-time measurement of mitochondrial membrane potential.
  • Investigation of mitochondria-shaping proteins' role in apoptosis.

Main Results:

  • Protocols were developed to study mitochondrial morphology and cell death.
  • Morphological changes in the mitochondrial reticulum were linked to cytochrome-c release.
  • Mitochondrial membrane potential changes were monitored in real time.

Conclusions:

  • Morphological dynamics of the mitochondrial reticulum are critical regulators of apoptosis.
  • The presented protocols enable quantitative assessment of mitochondrial involvement in cell death.
  • Understanding these mechanisms can inform therapeutic strategies for diseases involving cell death.