Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arabidopsis growth and reproduction require UDP-arabinofuranose import into the Golgi apparatus.

Plant physiology·2026
Same author

γ-Aminobutyric Acid (GABA): Metabolite, Messenger, and Mediator of Stress Adaptation.

Annual review of plant biology·2026
Same author

Continuous Directed Evolution of a Short-Lived Plant Histidinol Dehydrogenase.

ACS synthetic biology·2026
Same author

Harnessing mass spectrometry-based proteomics for continuous directed evolution.

Synthetic biology (Oxford, England)·2026
Same author

Metabolic Responses to Salinity Identify a Role for Mitochondrial 2-Oxoglutarate Dehydrogenase in Wheat Tissue Tolerance.

Plant, cell & environment·2025
Same author

Turnover rates of photosynthetic proteins indicate a correlation with photosynthetic capacity under state transitions.

Plant physiology·2025

Related Experiment Video

Updated: Jul 7, 2026

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
09:54

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Published on: January 5, 2018

Arabidopsis mitochondrial proteomics.

Joshua L Heazlewood1, A Harvey Millar

  • 1ARC Centre of Excellence in Plant Energy Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, Crawley.

Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2008
PubMed
Summary
This summary is machine-generated.

Researchers identified plant mitochondrial proteins using gel electrophoresis and mass spectrometry. This study provides a model for understanding plant mitochondrial proteomes.

More Related Videos

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
11:44

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Published on: September 28, 2013

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Related Experiment Videos

Last Updated: Jul 7, 2026

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
09:54

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Published on: January 5, 2018

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
11:44

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Published on: September 28, 2013

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Area of Science:

  • Plant biology
  • Mitochondrial research
  • Proteomics

Background:

  • Mitochondria are vital organelles in plant cells, crucial for energy production and metabolism.
  • Understanding the plant mitochondrial proteome is essential for advancing plant biology and agricultural science.
  • Arabidopsis thaliana serves as a key model organism for plant research.

Purpose of the Study:

  • To characterize the protein profile of Arabidopsis thaliana mitochondria.
  • To establish a foundational understanding of plant mitochondrial proteomes.
  • To present a reproducible methodology for plant mitochondrial protein identification.

Main Methods:

  • Mitochondrial protein extraction and separation using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE).
  • In-gel trypsination and complex protein lysate digestion for peptide generation.
  • Protein identification via matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry.

Main Results:

  • Detailed protein profiles of Arabidopsis mitochondria were obtained.
  • Successful identification of numerous mitochondrial proteins using mass spectrometry.
  • Validation of the combined proteomic techniques for plant mitochondria.

Conclusions:

  • The study successfully delineated the protein complement of Arabidopsis mitochondria.
  • The established methods serve as a robust model for plant mitochondrial proteome analysis.
  • This work contributes significantly to the field of plant mitochondrial biology and proteomics.