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Intracellular spatial control of fluorescent magnetic nanoparticles

Jinhao Gao1, Wei Zhang, Pingbo Huang

  • 1Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.

Journal of the American Chemical Society
|March 5, 2008
PubMed

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...