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Related Concept Videos

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Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Updated: Jul 7, 2026

Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
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Published on: October 17, 2025

Fiber-based confocal microscope for cryogenic spectroscopy.

Alexander Högele1, Stefan Seidl, Martin Kroner

  • 1Center for NanoScience, Department für Physik, Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, 80539 München, Germany. hoegele@phys.ethz.ch

The Review of Scientific Instruments
|March 5, 2008
PubMed
Summary
This summary is machine-generated.

A new fiber-based confocal microscope offers precise cryogenic positioning and stable optical performance. It enables advanced spectroscopy of quantum dots at low temperatures.

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Area of Science:

  • Cryogenics
  • Optical Microscopy
  • Quantum Dot Spectroscopy

Background:

  • Developing advanced microscopy techniques is crucial for studying quantum phenomena at low temperatures.
  • Existing cryogenic microscopes often face challenges with stability and precision.

Purpose of the Study:

  • To design and evaluate a fiber-based confocal microscope optimized for cryogenic operation.
  • To achieve high-precision positioning and stable optical performance at cryogenic temperatures.

Main Methods:

  • A fiber-based confocal microscope was designed with millimeter translation and nanometer precision positioning.
  • The system was tested at ambient, liquid nitrogen (77 K), and liquid helium (4 K) temperatures.
  • Stability was assessed under varying conditions, including temperature sweeps and magnetic fields up to 9 T.

Main Results:

  • The microscope demonstrated high stability and diffraction-limited optical performance across tested temperatures.
  • Its compact, nonmagnetic design ensured long-term position stability.
  • Successful application in single semiconductor quantum dot spectroscopy was shown.

Conclusions:

  • The developed cryogenic confocal microscope is a robust tool for low-temperature quantum research.
  • Its precision and stability make it suitable for advanced spectroscopic investigations.
  • This technology advances the study of quantum dots and similar nanomaterials.