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Related Experiment Videos

Ghrelin gets its GOAT.

James Gardiner1, Steve Bloom

  • 1Department of Investigative Medicine, Imperial College London, Du Cane Road, London W12 0NN, UK.

Cell Metabolism
|March 5, 2008
PubMed
Summary
This summary is machine-generated.

Ghrelin, an appetite hormone, requires an octanoyl side chain for activity. Researchers identified the enzyme ghrelin O-acyltransferase (GOAT) responsible for adding this crucial modification.

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Area of Science:

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Background:

  • Ghrelin is a key hormone regulating appetite and energy homeostasis.
  • Ghrelin's biological activity is dependent on acylation, specifically octanoylation at serine 3.
  • The enzyme responsible for this critical modification remained unidentified.

Purpose of the Study:

  • To identify the enzyme that catalyzes the octanoylation of ghrelin.
  • To elucidate the molecular mechanism of ghrelin activation.

Main Methods:

  • Utilized a combination of expression cloning and functional assays.
  • Employed techniques to detect ghrelin acylation and enzyme activity.

Main Results:

  • Successfully identified and cloned the enzyme responsible for ghrelin octanoylation.

Related Experiment Videos

  • Designated the enzyme as ghrelin O-acyltransferase (GOAT).
  • Demonstrated that GOAT is essential for ghrelin's ability to stimulate food intake in vivo.

    • Conclusions:

    • Ghrelin O-acyltransferase (GOAT) is the enzyme that activates ghrelin.
    • The discovery of GOAT provides a new target for understanding and treating metabolic disorders related to appetite regulation.