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Updated: Jul 7, 2026

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
Published on: August 26, 2016
Linking chemical extraction to microbial degradation of 14C-hexadecane in soil
Jacqueline L Stroud1, Graeme I Paton, Kirk T Semple
1Department of Environmental Science, Faculty of Science and Technology, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK.
Abstract:
Chemical extractions have been shown to measure the biodegradable fraction of aromatic contaminants in soil; however, there is little research on the chemical prediction of aliphatic hydrocarbon degradation. The aim of this study was to investigate the potential for cyclodextrin extractions to predict hexadecane biodegradation in soil. Soils were amended with 10 or 100 mg kg(-1) of a model alkane n-hexadecane and 100 Bq g(-1) (14)C-n-hexadecane. Correlations between the extents of mineralisation and extractions of the (14)C-contaminant were determined. Solvent shake extractions and aqueous CaCl(2) extractions were poor predictors of hexadecane bioaccessibility. However, the novel HP-alpha-CD shake extraction showed close correlation (r(2)=0.90, n=36, p<0.05) to the mineralisation data. This novel extraction technique has the potential to be used to assess the biodegradable aliphatic hydrocarbon fraction in contaminated soils.
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