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08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Genotyping of group A streptococcus by various molecular methods
Sobhan Nandi1, Nirmal K Ganguly, Rajesh Kumar
1Department of Experimental Medicine & Biotechnology, New Delhi, India.
The Indian Journal of Medical Research
|March 5, 2008
Summary
Random Amplification of Polymorphic DNA (RAPD) profiling is the most effective method for genotyping Group A Streptococcus (GAS) isolates in North India. This technique offers high discrimination and is comparable to emm typing for genetic analysis.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Group A Streptococcus (GAS) causes severe diseases in India.
- Genotypic characterization of GAS in India is limited.
- Understanding GAS genotypes is crucial for disease prevention and control.
Purpose of the Study:
- To investigate the genotype distribution of North Indian GAS isolates.
- To evaluate various molecular techniques for GAS genotyping.
- To identify reliable genetic markers for GAS identification.
Main Methods:
- Sixty clinical GAS isolates were analyzed.
- Molecular techniques included Restriction Enzyme Analysis (REA), ribotyping, PCR-ribotyping, and Random Amplification of Polymorphic DNA (RAPD).
- emm gene sequencing was used for comparative analysis.
Main Results:
- REA yielded six patterns; PCR-ribotyping resulted in three ribotypes.
- RAPD profiling provided the highest discrimination, identifying up to 12 distinct genetic profiles (rapdemes).
- Unique RAPD fragments were identified as potential genetic markers for GAS.
Conclusions:
- RAPD profiling is a highly discriminatory technique for genotyping GAS.
- The discriminatory power of RAPD is comparable to emm typing.
- This study provides valuable genotypic data for North Indian GAS isolates.
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