[New mutagen, glycidyl methacrylate (GMA) changed restriction enzyme map of plasmid]

Our present work showed that the mutant TcS ApR of plasmid pBR322 induced by GMA changed restriction enzyme map in the single and double enzyme digestions. Results indicated that the mutant plasmid has lots of mutated sites which are disappeared on some sites and appeared on others in resistance gene region, for example, sites of Bg I, EcoRI, HindIII and HincII, and occurred a new recognition site for HincII (252). The relationship between gene mutation and genotoxicity of GMA was discussed.