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Updated: Oct 9, 2026

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
Published on: June 14, 2014
[Chromosomal localization of human X chromosome alphoid satellite DNA by in situ hybridization and its preliminary
1Institute of Basic Medical Sciences, Beijing.
Abstract:
In situ hybridization of human X chromosome-specific alphoid satellite DNA to human lymphocyte metaphase and interphase nuclei was carried out. The results showed that the probe hybridized with high specificity to the centromeric region at p11----q11 of the X chromosome. The number of silver grain clusters in the interphase nuclei was correlated with that of X chromosomes in the cells. 44% of the clusters were located near the nuclear membrane, the usual location of X chromatin (Barr body). The hybridized chromosomes were also R-banded with BrdU treatment, and the significance of this work was briefly discussed.
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