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Tissue Triage and Freezing for Models of Skeletal Muscle Disease
Published on: July 15, 2014
Effects of freezing on bone histological morphology
Miguel Gustavo Setúbal Andrade1, Camila Neves Sá, Antônio Márcio Teixeira Marchionni
1Maxillofacial Surgery Division, Bahia Foundation for Science Development, Salvador, BA, Brazil. miguelsetubal@hotmail.com
Cell and Tissue Banking
|March 6, 2008
Summary
Cryopreservation of allograft bone for skeletal reconstruction alters its morphology. Freezing compromises bone tissue, especially at -70°C for extended periods, reducing antigenicity through cell necrosis.
Area of Science:
- Orthopedic Surgery
- Biomaterials Science
- Tissue Engineering
Background:
- Allograft bone is vital for skeletal reconstruction.
- Cryopreservation reduces allograft antigenicity.
- Morphological effects of cryopreservation on bone tissue are not fully understood.
Purpose of the Study:
- To investigate morphological changes in bone tissue after freezing.
- To evaluate effects of different temperatures and durations on bone morphology.
Main Methods:
- Rabbit bone tissue was frozen at -20°C and -70°C for 30-120 days.
- Histological (HE, picro-sirius, Feulgen's) and morphometric analyses were performed.
- Collagen expression and cellular/nuclear areas were quantified.
Main Results:
- Freezing increased cancellous bone cellular/nuclear areas but decreased cortical nuclear areas.
- Cortical bone collagen denatured with lower temperatures and longer freezing times.
- Significant morphological compromise observed after 90-120 days at -70°C.
Conclusions:
- Cryopreservation induces significant morphological alterations in allograft bone.
- Extended freezing at low temperatures (-70°C) negatively impacts bone tissue integrity.
- Cell necrosis during freezing contributes to reduced antigenicity, a key factor for allograft safety.
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