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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jul 6, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
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Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Super-resolution imaging by random adsorbed molecule probes.

Dongmin Wu1, Zhaowei Liu, Cheng Sun

  • 1NSF Nano-scale Science and Engineering Center, 3112 Etcheverry Hall, University of California, Berkeley, CA 94720, USA.

Nano Letters
|March 7, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a new super-resolution imaging technique using randomly adsorbed fluorescent probes on a metal substrate. This method enhances contrast by quenching signals, enabling super-resolution imaging without special fluorophores or object properties.

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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

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Last Updated: Jul 6, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Area of Science:

  • Optics
  • Nanotechnology
  • Biophysics

Background:

  • Single molecule localization (SML) achieves nanometer accuracy for molecular position and trajectory measurement.
  • Current SML super-resolution imaging relies on specialized photoactivable/photoswitchable fluorophores or diffusive probes.
  • Existing methods often require specific fluorophore or object properties, limiting broader applications.

Purpose of the Study:

  • To develop a new super-resolution technique applicable to bio/dielectric structures on metal substrates.
  • To overcome the limitations of current SML methods that require specialized fluorophores or object properties.
  • To enable super-resolution imaging for general dielectric objects without specific staining.

Main Methods:

  • Utilizing randomly adsorbed fluorescent probe molecules on a liquid-solid interface.
  • Employing a metal substrate to quench unwanted fluorescent signals, enhancing imaging contrast.
  • Achieving sub-diffraction-limited imaging without specialized fluorophores or object requirements.

Main Results:

  • Demonstrated a novel super-resolution technique for bio/dielectric structures on metal substrates.
  • Achieved significantly enhanced imaging contrast through substrate-induced fluorescence quenching.
  • Obtained sub-diffraction-limited images using randomly adsorbed probes.

Conclusions:

  • The proposed method offers a versatile super-resolution technique for various dielectric objects.
  • This approach eliminates the need for specific staining or specialized fluorophores.
  • Applicable to nanopatterned photoresist, inorganic nanowires, and subcellular structures.