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Related Experiment Videos

Fatty acids in human platelets and plasma. Fish oils decrease sensitivity toward N2 microbubbles.

A M Bakken1, M Farstad, H Holmsen

  • 1Laboratory of Clinical Biochemistry, University of Bergen, Norway.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|June 1, 1991
PubMed
Summary
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Marine oil supplements, rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), reduced N2 microbubble-induced platelet aggregation. This suggests potential benefits for divers and patients undergoing extracorporeal circulation.

Area of Science:

  • Cardiovascular Science
  • Marine Biology
  • Biochemistry

Background:

  • Microbubbles can form during deep diving and extracorporeal circulation.
  • Platelet aggregation is a key factor in thrombotic events.
  • Omega-3 fatty acids, EPA and DHA, are known for their anti-inflammatory and anti-aggregatory properties.

Purpose of the Study:

  • To investigate the effect of ethyl-EPA and ethyl-DHA supplementation on N2 microbubble-induced platelet aggregation.
  • To analyze changes in platelet and plasma fatty acid composition after supplementation.
  • To explore the correlation between specific fatty acids and microbubble-induced platelet aggregation.

Main Methods:

  • Seven volunteers received ethyl-EPA (3.5 g/day) and ethyl-DHA (2.5 g/day) for 2 weeks.

Related Experiment Videos

  • Platelet aggregation induced by N2 microbubbles was measured before and after supplementation.
  • Platelet and plasma fatty acid levels, including arachidonic acid (AA), EPA, and DHA, were analyzed.

    • Main Results:

    • Supplementation significantly decreased relative arachidonic acid (AA) content in platelets while increasing EPA and DHA.
    • Plasma EPA and DHA levels increased, with no significant change in AA.
    • N2 microbubble-induced platelet aggregation showed a significant negative correlation with platelet and plasma DHA content.
    • Higher platelet and plasma EPA levels were also associated with reduced aggregation.
    • A significant positive correlation was observed between platelet AA content and aggregation.

    Conclusions:

    • Ethyl-EPA and ethyl-DHA supplementation alters platelet and plasma fatty acid profiles, reducing AA and increasing omega-3s.
    • These changes are associated with decreased N2 microbubble-induced platelet aggregation.
    • Marine oil intake may offer protective benefits against microbubble-induced aggregation in high-risk scenarios like deep diving and extracorporeal circulation.