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The Skin Microbiota01:27

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The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
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Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
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Inter- and intra-individual variability in human skin barrier function: a large scale retrospective study.

Victor M Meidan1, Clive S Roper

  • 1Division of Pharmaceutical Sciences, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow G4 0NR, Scotland, UK. victor.meidan@strath.ac.uk <victor.meidan@strath.ac.uk>

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|March 7, 2008
PubMed
Summary

This study analyzed 2400 skin samples to establish reliable in vitro transepidermal water flux values. Our findings suggest revising the upper limit for tritiated water flux to enhance skin barrier integrity assessments.

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Area of Science:

  • Dermatology
  • Biophysics
  • Pharmacokinetics

Background:

  • In vitro transepidermal water flux measurements are crucial for assessing skin barrier integrity.
  • Previous studies relied on small sample sizes, limiting the statistical power of established flux values.

Purpose of the Study:

  • To conduct a large-scale statistical analysis of tritiated water permeability (kp) values.
  • To re-evaluate and potentially revise the established upper limits for tritiated water flux.
  • To investigate inter-individual and intra-individual variability in skin permeability.

Main Methods:

  • Retrospective statistical analysis of 2400 in vitro transepidermal tritiated water flux measurements.
  • Data collected from 112 female volunteers over a 4-year period.
  • Analysis of kp values, including distribution patterns and variability.

Main Results:

  • Tritiated water kp values followed a positively skewed, non-Normal distribution.
  • The mean kp was 2.04 x 10(-3)cm/h, and the 95th percentile was 4.50 x 10(-3)cm/h.
  • Intra-individual variability showed site-related differences, while inter-individual variability was normally distributed.

Conclusions:

  • Previously suggested upper limits for tritiated water flux are likely too low.
  • The upper limit for tritiated water flux should be revised to 4.5 x 10(-3)cm/h.
  • This revised limit will improve the accuracy of skin barrier integrity assessments in quality control.