Related Experiment Video
Updated: Jun 30, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
Trehalose-producing enzymes MTSase and MTHase in Anabaena 7120 under NaCl stress
Ravi K Asthana1, Subhasha Nigam, Archana Maurya
1Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, India. asthana.ravi@gmail.com
Abstract:
Salt tolerance, a multigenic trait, necessitates knowledge about biosynthesis and function of candidate gene(s) at the cellular level. Among the osmolytes, trehalose biosynthesis in cyanobacteria facing NaCl stress is little understood. Anabaena 7120 filaments exposed to 150 mM: NaCl fragmented and recovered on transfer to -NaCl medium with the increased heterocysts frequency (7%) over the control (4%). Cells failed to retain Na+ beyond a threshold [2.19 mM/cm3 (PCV)]. Whereas NaCl-stressed cells exhibited a marginal rise in K+ (1.1-fold) only at 30 h, for Na+ it was 130-fold at 48 h over cells in control. A time-course study (0-54 h) revealed reduction in intracellular Na+ beyond 48 h [0.80 mM/cm3 (PCV)] suggestive of ion efflux. The NaCl-stressed cells showed differential expression of maltooligosyltrehalose synthase (MTSase; EC 5.4.99.15) and maltooligosyltrehalose trehalohydrolase (MTHase; EC 3.2.1.141) depending on the time and the extent of intracellular Na+ buildup.
Related Concept Videos
Amino Acid Catabolism
Stringent Response in E. coli
Other Stress Responses in Bacteria

