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Related Experiment Video

Updated: Jul 6, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence.

F Dubois¹, L Joannes, J C Legros

¹Microgravity Research Center, Université Libre de Bruxelles, 50 Avenue F Roosevelt, CP165y62, B-1050 Brussels, Belgium. frdubois@ulb.ac.be

|March 8, 2008

Summary

This study introduces a digital holographic microscope for 3D imaging. It uses an incoherent light source and phase-stepping to achieve extended depth of investigation without mechanical refocusing.

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Area of Science:

Microscopy
Optical Engineering
3D Imaging

Background:

Laser-based digital holographic microscopy suffers from coherent noise.
Traditional microscopy requires mechanical adjustments for varying focal planes.

Purpose of the Study:

To develop a digital holographic microscopy technique for 3D imaging reconstruction.
To overcome limitations of coherent noise and mechanical focusing in microscopy.

Main Methods:

Implementation of a Mach-Zehnder interferometer with an incoherent light source.
Utilizing a phase-stepping technique for optical phase determination.
Employing digital holographic computations for refocusing out-of-focus planes.

Main Results:

Successfully reconstructed 3D images with enhanced depth of investigation.
Demonstrated the removal of coherent noise inherent in laser sources.
Provided experimental validation using a test target and biological samples.

Conclusions:

The digital holographic microscope offers a versatile, noise-reduced 3D imaging solution.
The technique significantly expands the depth of investigation without mechanical intervention.
Potential applications include particle localization and biological sample analysis.