Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defective queuosine and i6A/ms2i6A modification of tRNATyr cause frameshifting and protein aggregation.

Nucleic acids research·2026
Same author

Bandage-Type Autocatalytic PdCl<sub>2</sub>‑Containing Film as Visual Hydrogen Sensor for Noninvasive Monitoring of Mg-Alloy Biodegradation.

ACS applied optical materials·2026
Same author

Development of Universal Mass Exclusion List (UMEL) for RNA modification mapping.

RNA (New York, N.Y.)·2026
Same author

The effect of exercise self-efficacy on basic psychological needs in flight cadets: the chain mediating role of psychological resilience and perceived social support.

Frontiers in psychology·2026
Same author

Imaging and simulation study of electrokinetic supercharging in flow-gated capillary electrophoresis.

Analytical and bioanalytical chemistry·2025
Same author

Toward standardized epitranscriptome analytics: an inter-laboratory comparison of mass spectrometric detection and quantification of modified ribonucleosides in human RNA.

Nucleic acids research·2025
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: Jul 6, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Protein-aptamer binding studies using microchip affinity capillary electrophoresis.

Maojun Gong1, Irena Nikcevic, Kenneth R Wehmeyer

  • 1Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172, USA.

Electrophoresis
|March 8, 2008
PubMed
Summary
This summary is machine-generated.

Microchip capillary electrophoresis (CE) rapidly separates weak binding complexes, overcoming limitations of traditional methods. This technique accurately quantifies thrombin and its binding aptamer, enabling sensitive biomolecular analysis.

More Related Videos

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Related Experiment Videos

Last Updated: Jul 6, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Microfluidics

Background:

  • Traditional capillary electrophoresis (CE) struggles with weak binding complexes due to fast dissociation and nonspecific protein adsorption.
  • These issues complicate binding analysis and limit the study of dynamic biomolecular interactions.

Purpose of the Study:

  • To demonstrate the capability of microchip CE for studying weak binding systems, specifically thrombin and its aptamer.
  • To overcome the limitations of traditional CE methods for analyzing fast-dissociating complexes and reducing nonspecific adsorption.

Main Methods:

  • Utilized microchip CE with a short separation channel (1.0 cm) and high electric field (670 V/cm) for rapid separations.
  • Employed thrombin and a selective thrombin-binding aptamer as a model system.
  • Developed aptamer probes for quantitative analysis of thrombin samples.

Main Results:

  • Achieved rapid separation of thrombin-aptamer complex from free aptamer in under 10 seconds.
  • Determined a dissociation constant (Kd) of 43 nM for the thrombin-aptamer interaction, consistent with literature values.
  • Established a linear calibration curve for thrombin quantitation over two orders of magnitude, with a limit of detection (LOD) of 5 nM.

Conclusions:

  • Microchip CE is a powerful tool for analyzing weak binding systems, offering rapid separations and reduced nonspecific adsorption.
  • The method provides accurate determination of binding constants and sensitive quantitation of target analytes like thrombin.