Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Transcriptome analysis of grain development in hexaploid wheat.

Yongfang Wan¹, Rebecca L Poole, Alison K Huttly

¹Rothamsted Research, Harpenden, Hertfordshire, UK. yongfang.wan@bbsrc.ac.uk

|March 8, 2008

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Proteomic biomarkers differentiate bacterial infections in febrile infants: A multicentre prospective study.

The Journal of infection·2026

Same author

Vacuolar zinc transporter TaMTP1 safeguards wheat fertility and regulates grain zinc allocation via stem-mediated sequestration.

The Plant journal : for cell and molecular biology·2026

Same author

A single-centre randomised, feasibility study using point-of-care (POC) testing for respiratory viruses to direct oral corticosteroids use in preschool-aged children with acute wheeze: a protocol.

Pilot and feasibility studies·2026

Same author

Extreme heat and drought at flowering could threaten global wheat yields under climate change.

Climatic change·2026

Same author

INDETERMINATE DOMAIN-DELLA protein interactions orchestrate gibberellin-mediated cell elongation in wheat and barley.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Seed-type vacuolar processing enzymes recognize the 619th asparagine residue to posttranslationally cleave the HMW-GS 1Dy10-m619SN allele.

The Plant journal : for cell and molecular biology·2026

This study maps the wheat grain transcriptome, revealing distinct developmental phases and environmental impacts on gene expression. This data aids in understanding wheat development and improving crop traits.

Area of Science:

Plant Biology
Molecular Genetics
Agricultural Science

Background:

Hexaploid wheat (Triticum aestivum) is a crucial global food source.
Understanding grain development is key to enhancing yield and quality traits.
High-resolution transcriptomics offers powerful insights into plant biology.

Purpose of the Study:

To comprehensively analyze the transcriptome of developing wheat caryopses.
To identify gene expression patterns during distinct developmental stages.
To investigate the effects of environmental stress on wheat gene expression.

Main Methods:

Utilized Affymetrix wheat GeneChip oligonucleotide arrays for transcriptome profiling.
Analyzed transcript abundance in developing caryopses from 6 to 42 days after anthesis (daa).

More Related Videos

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

Related Experiment Videos

Last Updated: Jul 6, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

Confirmed key gene expression profiles using real-time RT-PCR.

Main Results:

Identified 14,550 differentially regulated transcripts during wheat grain development.
Characterized distinct transcriptomic phases: differentiation (6-10 daa), grain fill (12-21 daa), and desiccation/maturation (28-42 daa).
Showed environmental stresses primarily accelerate development, with some specific transcript responses.

Conclusions:

The generated transcriptome data serves as a valuable resource for wheat molecular research.
The study successfully differentiates developmental gene expression shifts from specific environmental treatment effects.
Expression profiles provide insights into the tissue specificity and function of wheat transcription factors.