Related Experiment Video
Updated: Jul 6, 2026
Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
A comparative study of selenium concentration and glutathione peroxidase activity in normal and breast cancer
Masoumeh Moradi1, Mohammad Hassan Eftekhari, Abdolrasoul Talei
1Department of Nutrition, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran. moradi_ma@yahoo.com
Objective:
The present study was undertaken to compare plasma Se values and glutathione peroxidase (GPX) activity in normal and breast cancer patients.
Design:
In a case-control study, forty-five breast cancer patients and the same number of healthy women were randomly selected from their population. Se was measured in plasma by atomic absorption spectrophotometry and GPX activity in erythrocytes was measured using a standard spectrophotometric method.
Results:
Plasma Se concentration in healthy women and breast cancer patients was in the normal range, with no statistically significant difference observed between the two groups (138.40 (sd 40.36) microg/l v. 132.15 (sd 35.37) microg/l, respectively). Erythrocyte GPX activity was significantly (P<0.01) higher in breast cancer patients (24.81 (sd 11.66) U/g Hb) compared with healthy women (20.29 (sd 4.24) U/g Hb).
Conclusion:
The present study indicated that Se deficiency was not a problem in the participants, and sufficient quantity of this element could increase GPX activity to have a protective effect against oxidative damage.
