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A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
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Ciprofloxacin-resistant Salmonella enterica serotype Typhimurium, China

Shenghui Cui1, Jingyun Li, Ziyong Sun

  • 1State Food and Drug Administration, Beijing, People's Republic of China.

Emerging Infectious Diseases
|March 8, 2008
PubMed

Insights

Multidrug-resistant Salmonella Typhimurium strains were identified in Wuhan, China. These resistant bacteria exhibited multiple antimicrobial drug resistance and carried specific genetic mutations linked to ciprofloxacin resistance.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Salmonella enterica serotype Typhimurium is a significant cause of bacterial gastroenteritis worldwide.
  • Antimicrobial resistance in Salmonella poses a growing public health threat.
  • Understanding resistance mechanisms is crucial for effective treatment and control.

Purpose of the Study:

  • To characterize Salmonella Typhimurium isolates from an outpatient population in Wuhan, China.
  • To investigate the antimicrobial resistance patterns and genetic basis of ciprofloxacin resistance.
  • To identify the presence of mobile genetic elements associated with resistance.

Main Methods:

  • Phenotypic characterization of 44 Salmonella Typhimurium isolates.
  • Antimicrobial susceptibility testing against multiple drugs.
  • Molecular analysis including gyrA and parC mutation detection.
  • Identification of class 1 integrons using PCR.

Main Results:

  • 31 out of 44 isolates were resistant to ciprofloxacin.
  • Ciprofloxacin-resistant isolates showed resistance to at least 8 other antimicrobial drugs.
  • All ciprofloxacin-resistant isolates harbored at least 2 mutations in gyrA and 1 mutation in parC.
  • Class 1 integrons were detected in 37 of the 44 isolates.

Conclusions:

  • Salmonella Typhimurium isolates from Wuhan exhibited extensive multidrug resistance.
  • Specific mutations in gyrA and parC are associated with high-level ciprofloxacin resistance.
  • The presence of class 1 integrons suggests a role in the dissemination of resistance genes.

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