Related Experiment Video
Updated: Jul 6, 2026

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
Published on: October 17, 2019
Divergent mechanisms in generating molecular variations of alphaRYR and betaRYR in turkey skeletal muscle
Wen Chiang1, Hyo-Jung Yoon, John E Linz
1Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI, 48824, USA. wen.chiang@rdmo.nestle.com
Abstract:
(1) Molecular variations in two turkey skeletal muscle ryanodine receptor gene isoforms, alphaRYR and betaRYR, were analyzed by cloning and sequencing the entire cDNAs of the two isoforms. (2) Ten alternative splicing transcript variants (ASTVs) in the alphaRYR isoform were identified. These variants were clustered in three alternative splicing regions (ASRs). Two ASRs overlap with the divergent regions (DRs) of the two isoforms. Only four ASTVs did not contain a frame shift and potentially can be translated into alphaRYR channel proteins. The expression of these three ASTVs was developmentally or environmentally regulated. (3) Ten SNPs and eight haplotypes, divergent in the ten SNP positions, were identified in betaRYR. Although the ten SNPs were synonymous, different mRNA secondary structures of betaRYR and different stability of the structures were predicted for several SNPs. (4) The intriguing finding of this study is that alphaRYR and betaRYR use completely divergent mechanisms to generate molecular variations. Alternative splicing generates ASTVs of alphaRYR, whereas the presence of SNPs may change the secondary mRNA structure of betaRYR. These divergent mechanisms could affect calcium channel activity of either or both RYR isoforms.
Related Concept Videos
Multiple Allele Traits
Genetic Variation
Genes exist in different versions called alleles, which...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Epistasis Analysis
Classification of Skeletal Muscle Fibers
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

