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Updated: Apr 28, 2026
Murine Skin Transplantation
Published on: January 16, 2008
[Effect of anisomycin on allogeneic skin transplantation in mice]
Di Chen1, Fei-yue Xing, Jing-fang DI
1Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
Aim:
To investigate the effect of anisomycin on the reactivity of lymphocytes and the rejection in the skin transplantation in mice.
Methods:
The reactivity of T cells in unilateral or bilateral mixed lymphocyte reaction was detected by MTT assay. The skins from C57BL/6 mice were transplanted to BALB/c mice and the survival period of the grafts was assessed. The recipients were divided into three groups (physiological saline group, cyclosporin A group and anisomycin group). They were injected with physiological saline (0.1 mL), cyclosporin A (20 mg/kg) and anisomycin (12.5 mg/kg) separately once a day for 3 days before the operation and 7 days after the operation.
Results:
With the optimal dose of 10.0 microg/L, anisomycin inhibited the reactivity of lymphocyte cells in both unilateral and bilateral mixed lymphocyte reactions. The survival period of the grafts from anisomycin group was longer than that from cyclosporin A group and physiological saline group.
Conclusion:
Anisomycin can inhibit the reaction of lymphocytes and prolong the survival period of the grafts in skin transplantation effectively. It could be used as a new way for transplantation rejection treatment.
