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Updated: Jul 6, 2026

Reconstitution of Actin-Based Motility with Commercially Available Proteins
Published on: October 28, 2022
How do in vitro reconstituted actin-based motility assays provide insight into in vivo behavior?
Beáta Bugyi1, Christophe Le Clainche, Guillaume Romet-Lemonne
1Cytoskeleton Dynamics and Motility Group, LEBS, UPR 3082, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
Abstract:
Recent live cell image analysis of actin dynamics in lamellipodia of motile cells has shown that regulated treadmilling, which supports actin-based propulsion of functionalized particles in biomimetic reconstituted motility assays, is also responsible for lamellipodia extension. In both cases, filaments are created by branching with Arp2/3 complex only at the membrane or particle surface, grow transiently and are capped; ADF/cofilin enhances the treadmilling but does not sever filaments in the body of the meshwork. Differences between the cellular and biomimetic systems suggest that additional regulatory mechanisms take place in lamellipodia.
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