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Updated: Jul 6, 2026

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

Published on: November 17, 2016

Sutureless amniotic membrane transplantation for partial limbal stem cell deficiency.

Ahmad Kheirkhah¹, Victoria Casas, Vadrevu K Raju

¹Ocular Surface Center, Miami, Florida 33173, USA.

American Journal of Ophthalmology

|March 11, 2008

Summary

Sutureless amniotic membrane transplantation with fibrin glue effectively reconstructs the corneal surface in partial limbal stem cell deficiency (LSCD). This safe method restores stable corneal epithelium without needing limbal stem cell transplantation.

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Area of Science:

Ophthalmology
Regenerative Medicine
Corneal Surgery

Background:

Partial limbal stem cell deficiency (LSCD) compromises corneal integrity and visual function.
Reconstructing the corneal surface in LSCD is crucial for vision restoration.
Current treatments may involve complex procedures or stem cell transplantation.

Purpose of the Study:

To evaluate the efficacy and safety of sutureless amniotic membrane (AM) transplantation using fibrin glue for partial LSCD.
To assess the outcomes of AM transplantation in patients with varying degrees of limbal involvement.
To determine if this method can avoid the need for limbal epithelial stem cell transplantation.

Main Methods:

Retrospective analysis of eleven eyes from nine patients with partial LSCD.

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Published on: October 27, 2023

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Superficial keratectomy followed by sutureless AM transplantation using fibrin glue.

Use of additional AM patch and mitomycin C in select cases; repeat surgeries for residual pannus.

Main Results:

Stable corneal epithelial surface achieved in all eyes during a mean 14.2-month follow-up.
Significant improvement in best-corrected visual acuity in 81.8% of eyes.
Reduced stromal cloudiness and vascularization; no complications related to fibrin glue.

Conclusions:

Sutureless amniotic membrane transplantation with fibrin glue is a safe and effective treatment for partial LSCD.
This technique successfully restores a stable corneal epithelium.
The procedure offers an alternative to limbal epithelial stem cell transplantation.