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Insulin Formulations: Types and Delivery

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Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

Functionalized injectable hydrogels for controlled insulin delivery.

Dai P Huynh¹, Minh K Nguyen, Bong S Pi

¹Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi 440-746, Republic of Korea.

|March 11, 2008

Summary

This study introduces a novel injectable hydrogel using poly(beta-amino ester) for controlled drug delivery. The pH/temperature-sensitive hydrogel forms ionic complexes with biomolecules like insulin, enabling tunable release kinetics.

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Area of Science:

Biomaterials Science
Polymer Chemistry
Drug Delivery Systems

Background:

Developing injectable hydrogels for controlled drug and protein delivery is crucial for therapeutic applications.
Existing hydrogels often lack the sensitivity and functionality required for precise release modulation.
Poly(beta-amino ester) (PAE) offers unique pH-sensitive and cationic properties suitable for advanced biomaterial design.

Purpose of the Study:

To synthesize a novel pH/temperature-sensitive injectable hydrogel using a pentablock copolymer PAE-PCL-PEG-PCL-PAE.
To utilize the dual functionality of PAE for forming ionic complexes with anionic biomolecules, enabling controlled release.
To investigate the in vitro and in vivo release kinetics of insulin from the developed hydrogel system.

Main Methods:

Synthesis of PAE-PCL-PEG-PCL-PAE pentablock copolymer by conjugating PAE to a biodegradable triblock copolymer.
Investigation of sol-gel phase transition behavior in response to pH and temperature.
In vitro and in vivo studies to evaluate insulin complexation, degradation, and release profiles.
Simulation of ionic complex formation between PAE and anionic biomolecules.

Main Results:

The pentablock copolymer exhibited pH and temperature-dependent sol-gel phase transitions.
Ionic complexation with insulin was successfully achieved, influencing hydrogel properties and degradation.
In vitro and in vivo experiments demonstrated controlled release of insulin, with improved pharmacokinetic profiles compared to control hydrogels.
Simulations provided insights into the interaction mechanism between PAE and insulin.

Conclusions:

The PAE-based pentablock copolymer hydrogel is a promising platform for sensitive and controlled delivery of biomolecules like insulin.
The dual functionality of PAE allows for tunable release mechanisms based on hydrogel degradation and ionic interactions.
This injectable hydrogel system holds potential for advanced therapeutic applications requiring precise drug release.