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Updated: Jul 6, 2026
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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
[The analysis of sinusoidal modulated method used for measuring fluorescence lifetime]
1Institute of Optoelectronics and Technology, Beijing Jiaotong University, Key Laboratory of Luminescence and Optical Information Ministry of Education, Beijing 100044, China.
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|March 12, 2008
Summary
Researchers developed a system to measure the fluorescence lifetime of Europium (Eu3+) ions, achieving a precise 0.680 ms result. This advanced phase-shift method improves accuracy for complex fluorescent materials.
Area of Science:
- Luminescence and Spectroscopy
- Materials Science
- Analytical Chemistry
Context:
- Investigating the photophysical properties of rare-earth ions is crucial for developing advanced optical materials.
- Accurate fluorescence lifetime measurements are essential for characterizing luminescent materials and their applications.
- The phase-shift method is a standard technique for fluorescence lifetime determination.
Purpose:
- To develop and validate a system for precise fluorescence lifetime measurement of Eu3+ ions.
- To enhance the phase-shift method for handling complex fluorescence decay and modulation harmonics.
- To determine the fluorescence lifetime of Eu2 L'3 x nH2O (L' = C4H4O4) samples.
Summary:
- A sinusoidal modulated LED excitation source was employed to measure the excitation light and 5Do-7F2 emission of Eu3+ ions in Eu2 L'3 x nH2O.
- Fluorescence lifetime was determined using non-linear least square curve fitting based on phase-shift measurement principles.
- Advanced data processing methods, including Fourier series expansion, were utilized to account for high-order harmonics and multi-exponential decay, improving accuracy.
Impact:
- The study successfully determined a fluorescence lifetime of approximately 0.680 ms for the Eu3+ ion.
- The developed data processing techniques expand the applicability of the phase-shift method for more complex systems.
- This research contributes to more accurate characterization of luminescent materials, enabling improved design for optical and sensing applications.
