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Updated: Jul 6, 2026

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
Published on: April 6, 2012
Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue
Hwan Myung Kim1, Byeong Ha Jeong, Ju-Yong Hyon
1Department of Chemistry, National Creative Research Initiative Center for Cell Dynamics, Department of Physics, Biomicrosystems Technology Program, Korea University, 1-Anamdong, Seoul, 136-701, Korea.
Abstract:
We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy.
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