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Updated: Jul 6, 2026

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants
Published on: March 11, 2013
The particles of the embryonic cerebrospinal fluid: how could they influence brain development?
Isabelle Bachy1, Renata Kozyraki, Marion Wassef
1CNRS UMR 8542, Ecole Normale Supérieure, 75005 Paris, France. bachy@biologie.ens.fr
Abstract:
During brain development, the embryonic cerebrospinal fluid (E-CSF) allows brain expansion and promotes neuroepithelial cell survival, proliferation or differentiation. Previous analyses of E-CSF content have revealed a high protein concentration and the presence of membranous particles. The role of these particles in the E-CSF remains poorly investigated. In this study we showed that the E-CSF contains at least two pools of particles: lipoproteins and exosome-like particles. We showed that these two populations of particles strongly interact with neuropithelial cells via an endocytic process, which display regional specificity along the developing neural tube. Finally, we explore and discuss the possibility that these interactions may influence brain development through the regulation of morphogen and growth factor signaling transduction.
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