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Related Experiment Videos

Purification and characterization of scatter factor.

E Gherardi1, A Coffer

  • 1Department of Medicine, Cambridge University, Addenbroke's Hospital, England.

EXS
|January 1, 1991
PubMed
Summary

Scatter factor, a fibroblast-derived protein, enhances cell movement and disrupts epithelial colonies. This protein, crucial for mesenchymal-epithelial interactions, is closely related to hepatocyte growth factor.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Fibroblast-derived scatter factor influences epithelial cell behavior.
  • This protein enhances the motility of epithelial and endothelial cells.
  • It also disrupts epithelial colonies, indicating a role in tissue dynamics.

Purpose of the Study:

  • To characterize the biochemical properties of mouse fibroblast scatter factor.
  • To investigate its structure, activity, and relationship with other growth factors.
  • To understand its role in mesenchymal-epithelial interactions.

Main Methods:

  • Purification using cation-exchange and reverse-phase chromatography.
  • Molecular weight determination via SDS-PAGE, gel filtration, and ultracentrifugation.
  • Analysis of biochemical properties including heparin-binding, basicity (pI), and protease activity.

Main Results:

  • Mouse scatter factor is a 61.4 kD dimer composed of 57 kD and 30 kD subunits.
  • It is active at picomolar concentrations and requires intact disulfide bonds.
  • The protein is heparin-binding, basic, and contains non-essential N-linked carbohydrates.
  • No metallo- or serine protease activity was detected.

Conclusions:

  • Mouse scatter factor is a potent effector of mesenchymal-epithelial interactions.
  • It is identical or closely related to hepatocyte growth factor/hepatopoietin A.
  • Its junctional-breaking activity does not appear to involve proteolytic cleavage.

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